The detection principle of the Bradford method is that Coomassie Brilliant Blue G-250 binds to the basic and aromatic amino acids of the protein, especially arginine, in an acidic medium, at which point the solution turns blue, and the maximum absorption peak of the solution migrates from 465 nm to 595 nm. The protein concentration in the solution can be quantitatively determined by detecting the absorbance value at 595 nm, according to the change in the color of the solution is proportional to the protein concentration.

The Bradford Protein Assay Kit is developed according to the Bradford method and is one of the commonly used methods for protein quantification, which can achieve fast, stable and highly sensitive protein concentration determination with good linearity of detection data.

The kit has the following advantages: extremely fast detection speed, compared with BCA, 10-20 samples can be completed in less than 10 minutes, greatly reducing the detection time, high detection sensitivity, 0.5 μg protein detection, good standard curve linearity, 5 μL volume of samples or standards have a good linear relationship in the concentration range of 0.1-1.5 mg/mL.