Quantibody® Bovine Cytokine Array 1 Kit. Detects 10 Bovine Cytokines. Suitable for all liquid sample types.
Bovine Cytokine Array Q1
Bovine Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Bovine (cow) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| IFN-alpha A
| IFN-gamma
| IL-13
| IL-1 alpha (IL-1 F1)
| IL-1 F5 (FIL1 delta)
|
| IL-21
| IP-10 (CXCL10)
| MIG (CXCL9)
| MIP-1 beta (CCL4)
| TNF alpha
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Quantibody® Bovine Cytokine Array 1 Slide
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAB-CYT-1.gal)
Excel (QAB-CYT-1_Analysis_Tool.xls)
citations
Rumen microbiota modulates metabolic stress in high-yield dairy cows: insights from early to peak lactation
- Species:
- Bovine
- Sample Type:
- Serum (early to peak lactation dairy cows)
- Year:
- 2026
- Journal Name:
- Microbiome
- PubMed ID:
- 41514445
Proteomics and cytokine array jointly reveal the role of macrophage proinflammatory shift in liver fibrosis in dairy cows with ketosis
- Species:
- Bovine
- Sample Type:
- Tissue Lysate / Homogenate (liver tissue)
- Year:
- 2025
- Journal Name:
- Journal of animal science and biotechnology
- PubMed ID:
- 40629398
Influence of differing levels of concentrate on circulating cytokine concentrations in beef heifers
- Species:
- Bovine
- Sample Type:
- Plasma
- Year:
- 2024
- Journal Name:
- Translational animal science
- PubMed ID:
- 38873027
Dairy Cows Experimentally Infected With Bovine Leukemia Virus Showed an Increased Milk Production in Lactation Numbers 3–4: A 4-Year Longitudinal Study
- Species:
- Bovine
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Frontiers in microbiology
31 Correlation of the Reproductive Microbiome and Cytokine Profiles with Plasma Progesterone Concentrations in Postpartum Beef Cows Prior to Timed Artificial Insemination
- Species:
- Bovine
- Sample Type:
- Plasma, uterine flushes
- Year:
- 2023
- Journal Name:
- Journal of Animal Science
An updated profile of the bovine acute phase response following an intravenous lipopolysaccharide challenge
- Species:
- Bovine
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of Animal Science
Reproductive microbiome and cytokine profiles associated with fertility outcomes of postpartum beef cows
- Species:
- Bovine
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Journal of Animal Science
PSIII-14 Reproductive Microbiome and Cytokine Profiles of Postpartum Beef Cows in Relation to Fertility
- Species:
- Bovine
- Sample Type:
- Blood
- Year:
- 2022
- Journal Name:
- Journal of Animal Science
PSV-B-16 Correlation between Plasma, Uterine, and Vaginal Cytokine Concentrations in Postpartum Beef Cows Prior to Artificial Insemination
- Species:
- Bovine
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Journal of Animal Science
Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle
- Species:
- Bovine
- Sample Type:
- Plasma, follicular fluid (FF)
- Year:
- 2023
- Journal Name:
- Animals