Anti-TMBIM1 Antibody

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Size : 50ul

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Western blot analysis of TMBIM1 expression in A549 (A), MCF7 (B), COS7 (C), CT26 (D), PC12 (E) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 35 kD)
Immunohistochemical analysis of TMBIM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TMBIM1 staining in BV2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-TMBIM1 Antibody
Cat No:	CPA4278
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to TMBIM1

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human TMBIM1. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of TMBIM1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/50 - 1/200)

Gene Symbol：TMBIM1

Alternative Names：LFG3; RECS1; Protein lifeguard 3; Protein RECS1 homolog; Transmembrane BAX inhibitor motif-containing protein 1

Entrez Gene (Human)： 64114;

Entrez Gene (Mouse)： 69660;

SwissProt (Human)： Q969X1;

SwissProt (Mouse)： Q8BJZ3;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.