Anti-SP6 Antibody

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Size : 200ul

Phone : +1 850 650 7790

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Phone : +1 850 650 7790

Western blot analysis of SP6 expression in K562 (A) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD)
Flow cytometric analysis of K562 cells using Anti-SP6 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Product Name:	Anti-SP6 Antibody
Cat No:	CQA7063
Source:	Rabbit
Reactivity:	H
Applications:	WB, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to SP6

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human SP6. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of SP6 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), FC (1/10 - 1/50)

Gene Symbol：SP6

Alternative Names：KLF14; Transcription factor Sp6; Krueppel-like factor 14

Entrez Gene (Human)： 80320;

SwissProt (Human)： Q3SY56;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.