Anti-Rat IL-4
Cat# I-225-25
Size : 25ug
Brand : Leinco Technologies
Contact local distributor :
Phone : +1 850 650 7790
AntiRat IL4
AntiRat IL4
Product No.: I225
Target IL4 Product Type Polyclonal Antibody Alternate Names Interleukin4, BCGF, BCDF, B Cell Stimulating Factor, BSF1 Applications N , WB |
Antibody DetailsProduct DetailsReactive Species Rat Host Species Goat Immunogen Purified Recombinant Rat IL4 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830577 Applications and Recommended Usage? Quality Tested by Leinco Western Blot: Suitable for use at concentration of 0.1 µg/mL. Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize IL‑4induced proliferation in the rat splenocytes. The Neutralization Dose (ND50) is typically 0.05015 µg/mL in the presence of 4 ng/mL Recombinant Rat IL‑4 and 10 µg/mL PHA. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rabbit AntiRat Interleukin4 (IL4) recognizes Rat IL4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background IL4 is a 1519 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated Bcell and Tcell proliferation, and initiation of allergic responses. Moreover, IL4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL4. Increased IL4 production and overproduction of IL4R are found in many cancers. IL4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL4 may also play a part in the immune dysregulation that occurs in most HIV1 infected patients. PubMed NCBI Gene Bank ID UniProt.org Research Area Other Molecules References & Citations1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551 |