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> Anti-p53 (Phospho-S6) Antibody

Anti-p53 (Phospho-S6) Antibody

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Cat# CPA4852-100ul

Size : 100ul

Brand : Cohesion Biosciences

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of p53 (Phospho-S6) expression in A549 (A), HEK293T (B), rat kidney (C), mouse kidney (D) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53; 47 kD)
  • Immunohistochemical analysis of p53 (Phospho-S6) staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of p53 (Phospho-S6) staining in SVHUC1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Direct ELISA antibody dose-response curve using Anti-p53 (Phospho-S6) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
Product Name:Anti-p53 (Phospho-S6) Antibody
Cat No:CPA4852
Source:Rabbit
Reactivity:H, M, R, Mk
Applications:WB, IH, IF/IC, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to p53 (Phospho-S6)
Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S6 of human p53 protein. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of p53 protein only when phosphorylated at S6.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/50 - 1/200), IP (1/10 - 1/100)
Gene Symbol：TP53
Alternative Names：P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Entrez Gene (Human)： 7157;
SwissProt (Human)： P04637;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.