This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

This purified antibody is stable when stored at 28°C. Do not freeze.

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Clone TR7589 recognizes mouse CD120b.

CD120 can refer to two members of the tumor necrosis factor receptor superfamily CD120a (TNFR1) or CD120b (TNFR2). CD120a is a 55kD Type I transmembrane protein receptor that binds both TNFα and TNFβ (LTα). In association with TRADD and RIP, the receptor crosslinking induced by TNFα or TNFβ trimers is vital for signal transduction, leading to apoptosis, NFB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. CD120b is a 75 kD type I transmembrane protein that binds both TNFα and TNFβ. In conjunction with TRAF1 and TRAF2, the receptor crosslinking induced by TNFα or TNFβ trimers is critical for signal transduction that may lead to apoptosis, NFkB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. TNFα is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNFα secreted by the macrophage causes the blood to clot which provides containment of the infection. TNFα binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. TNFβ (LTα) is a 25 kD protein that has a significant impact on the maintenance of the immune system including the development of secondary lymphoid organs. TNFβ has dual functions. It may function to prevent growth of cancer cells or it may facilitate the development of tumors. TNFβ is involved in the regulation of cell survival, proliferation, differentiation, and apoptosis and, if unregulated, can result in a constantly active signaling pathway, resulting in uncontrolled cellular growth and creation of tumors. Additionally, TNFβ is involved in innate immune regulation and has been shown to prevent tumor growth and obliterate cancerous cell lines.

TNFR2 is expressed by a variety of cell types including hematopoietic cells such as macrophages, neutrophils, lymphocytes, thymocytes and mast cells.

1. Zuckerman, KS. et al. (1998) Cancer Res. 58:2217.
2. Sheehan, KC. et al. (1995) J. Exp. Med. 181:607.