Anti-MFN1 Antibody

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Size : 50ul

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Western blot analysis of MFN1 expression in A549 (A), mouse liver (B), rat brain (C) whole cell lysates. (Predicted band size: 41; 71; 84 kD; Observed band size: 84 kD)
Immunofluorescent analysis of MFN1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-MFN1 Antibody
Cat No:	CQA3646
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to MFN1

Immunogen：Recombinant full length protein of human MFN1

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of MFN1 protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Gene Symbol：MFN1

Alternative Names：Mitofusin-1; Fzo homolog; Transmembrane GTPase MFN1

Entrez Gene (Human)： 55669;

Entrez Gene (Mouse)： 67414;

Entrez Gene (Rat)： 192647;

SwissProt (Human)： Q8IWA4;

SwissProt (Mouse)： Q811U4;

SwissProt (Rat)： Q8R4Z9;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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