Reagents and instruments for immunology, cell biology and molecular biology.
 
> Anti-eNOS (Phospho-S1177) Antibody

Anti-eNOS (Phospho-S1177) Antibody

Print Print
Please log in

Cat# CPA1818-50ul

Size : 50ul

Brand : Cohesion Biosciences

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of eNOS (Phospho-S1177) expression in A549 (A), HuvEc (B), rat brain (C) whole cell lysates. (Predicted band size: 133; 69 kD; Observed band size: 140 kD)
  • Immunofluorescent analysis of eNOS (Phospho-S1177) staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-eNOS (Phospho-S1177) Antibody
Cat No:CPA1818
Source:Rabbit
Reactivity:H, M, R, B, D, P, Rb, S
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to eNOS (Phospho-S1177)
Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S1177 of human eNOS protein. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of eNOS protein only when phosphorylated at S1177.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol：NOS3
Alternative Names：Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Entrez Gene (Human)： 4846;
Entrez Gene (Mouse)： 18127;
Entrez Gene (Rat)： 24600;
SwissProt (Human)： P29474;
SwissProt (Mouse)： P70313;
SwissProt (Rat)： Q62600;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.