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> Anti-Cytochrome C/CYCS Antibody Picoband®

Anti-Cytochrome C/CYCS Antibody Picoband®

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Cat# PA1118

Size : 100ug/vial

Brand : Boster Bio

Contact local distributor :


Phone : +1 850 650 7790

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Tested Applications

CYCS antibody was positively detected by WB in:

human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human HT1080 whole cell, rat kidney tissue, rat skeletal muscle tissue, mouse kidney tissue, mouse skeletal muscle tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CYCS antibody was positively detected by IHC in:

human skeletal muscle tissue, rat kidney tissue, human liver tissue, rat kidney tissue, human appendix tissue, human liver tissue, human colon tissue, human cervial tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CYCS antibody was positively detected by ICC/IF in:

A431 cell

CYCS antibody was positively detected by IF in:

human cervical cancer tissue, human liver cancer tissue, human colon cancer tissue, rat heart tissue, mouse heart tissue

CYCS antibody was positively detected by FC in:

CACO-2 cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat, Mouse
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human, Rat, Mouse
Immunocytochemistry
0.5-1μg/ml
Human, -
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-μg/1x106 cells
Human

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Product Information

Product Name
Anti-Cytochrome C/CYCS Antibody Picoband®
View all CYCS Antibodies
Form
Lyophilized
Uniprot
P99999
Gene ID
54205
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
14 kDa
Calculated Molecular Weight

11.7 kDa

Background of CYCS
Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system. Human cytochrome c has 104 amino acid residues and a molecular weight of 11,458 and is mapped to 7p15.2. Cytochrome c released from mitochondria has been proposed to be an essential component of an apoptotic pathway responsive to DNA damage and other forms of cell stress. And it has a role in different apoptotic signaling cascades.
Cite This Product
Anti-Cytochrome C/CYCS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1118)

Protocols

Western Blot protocol for CYCS antibody
Review Protocol
Immunohistochemistry (IHC) protocol for CYCS antibody
Review Protocol
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CYCS antibody
Review Protocol
Immunofluorescence (IF) protocol for CYCS antibody
Review Protocol
Flow Cytometry (FC) protocol for CYCS antibody
Review Protocol

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