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> Anti-Collagen III/COL3A1 Antibody Picoband® FITC Conjugated

Anti-Collagen III/COL3A1 Antibody Picoband® FITC Conjugated

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Cat# A00788-3-FITC

Size : 100ug/vial

Brand : Boster Bio

Contact local distributor :


Phone : +1 850 650 7790

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a00788 3 col3a1 primary antibodies ihc testing 2
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a00788 3 col3a1 primary antibodies ihc testing 4
a00788 3 col3a1 primary antibodies ihc testing 5
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Tested Applications

COL3A1 antibody was positively detected by WB in:

mouse skin tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

COL3A1 antibody was positively detected by IHC in:

human gastric poorly differentiated adenocarcinoma tissue, human tonsil tissue, human liver cancer tissue, human lung adenocarcinoma tissue, mouse lung tissue, rat lung tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

COL3A1 antibody was positively detected by FC in:

Hela cell

Recommend Dilution

Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
ELISA
0.1-0.5μg/ml

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.

Product Information

Product Name
Anti-Collagen III/COL3A1 Antibody Picoband®
View all COL3A1 Antibodies
Form
Lyophilized
Uniprot
P02461
Gene ID
1281
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).
Cross-Reactivity
No cross reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
200 kDa
Calculated Molecular Weight

138.6 kDa

Background of COL3A1
COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Cite This Product
Anti-Collagen III/COL3A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00788-3)

Protocols

Western Blot protocol for COL3A1 antibody
Review Protocol
Immunohistochemistry (IHC) protocol for COL3A1 antibody
Review Protocol
Flow Cytometry (FC) protocol for COL3A1 antibody
Review Protocol

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