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> Anti-CD154 Antibody

Anti-CD154 Antibody

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Cat# CPA1186-30ul

Size : 30ul

Brand : Cohesion Biosciences

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Phone : +1 850 650 7790

  • Western blot analysis of CD154 expression in K562 (A), SP20 (B), Myla2059 (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 36 kD)
  • Immunofluorescent analysis of CD154 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-CD154 Antibody
Cat No:CPA1186
Source:Rabbit
Reactivity:H, M, R, B, D, P, Rb, S
Applications:WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to CD154
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD154. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Specificity：Recognizes endogenous levels of CD154 protein.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol：CD40LG
Alternative Names：CD40L; TNFSF5; TRAP; CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154
Entrez Gene (Human)： 959;
Entrez Gene (Mouse)： 21947;
Entrez Gene (Rat)： 84349;
SwissProt (Human)： P29965;
SwissProt (Mouse)： P27548;
SwissProt (Rat)： Q9Z2V2;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.