Anti-CBP | CREB-binding protein homolog

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Cat# AS132730

Size : 50ug

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Phone : +1 850 650 7790

Brand : Agrisera

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Contact local distributor :

Phone : +1 850 650 7790

Anti-CBP | CREB-binding protein homolog

Product no: AS13 2730

AS13 2730 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Drosophila melanogaster

Immunogen:	Recombinant CBP part, fused to GST, derived from Drosophila melanogaster protein sequence, UniProt: O01368
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
Reconstitution:	For reconstitution add 50 µl of sterile water
Storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	3 µg (ChIP), 1 : 500 (WB)
Expected \| apparent MW:	331 kDa

Confirmed reactivity:	Drosophila melanogaster
Predicted reactivity:	Drosophila melanogaster
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

application example

Total nuclear protein from 2x10^6 Drosophila melanogaster cultured cells was separated on 6% SDS-PAGE and blotted to PVDF using tank transfer. The blot was incubated in the primary antibody at a dilution of 1: 500 in 1% BSA, 0.05% Tween-20, 1x PBS for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice with 1x PBS. Blot was incubated in secondary antibody (anti-rabbit IgG AP-conjugated) diluted to 1:10 000 in 1% BSA, 0.05% Tween-20, 1x PBS for 30 min at RT with agitation. The blot was rinsed twice with 1x PBS and equilibrated in detection buffer (100mM NaCl; 10mM Tris-HCl pH9.5; 10mM MgCl2). The blot was incubated in substrate solution: 165ug/ml of BCIP and 330ug/ml of NBT in detection buffer until the signal reached the desired contrast and then rinsed with water and air dried.

Cortesy of Dr. Tatyana Kahn, Umeå University, Sweden

Background:

CBP (CREB-binding protein homolog) is a transcriptional coactivator in multiple signal transduction pathways. It might play a role in variety of cellular processes, including differentiation, cellular proliferation, immune response, homeostasis, tumorigenesis, and erganogenesis.

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