Specifications

Product Data
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	TrueORF Clone RC205708
Tag	C-Myc/DDK
Detection Antibodies	Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN	NM_020376, NP_065109
Synonyms	1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; TTS-2.2; TTS2
Predicted MW	55.3 kDa
Components	1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage	The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping	Dry Ice
Preparation	HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq	NP_065109
Locus ID	57104
Cytogenetics	11p15.5
Protein Families	WB

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Over-expression Cell Lysate

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SDS for LY402777

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH305708	PNPLA2 MS Standard C13 and N15-labeled recombinant protein (NP_065109)	10 ug
LC402777	Lyophilized PNPLA2 HEK293T cell transient overexpression lysate (as WB positive control)	20 ug
TP305708	Recombinant protein of human patatin-like phospholipase domain containing 2 (PNPLA2), 20 µg	20 ug