ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:1000

ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa].
Green : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature