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> α-Tocopherol (phosphate) [38976-17-9]

α-Tocopherol (phosphate) [38976-17-9]

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Cat# HY-16686-5mg

Size : 5mg

Brand : MedChemExpress

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Description

α-Tocopherol phosphate is an antioxidant that protects against long-wave UVA1 induced cell death and scavenge UVA1 induced ROS in a skin cell model. α-Tocopherol phosphate exhibits angiogenesis-promoting activity[1][2].

In Vitro

α-Tocopherol phosphate (100 μM, 24 h) inhibits high Glucose (HY-B0389)/ischemic-induced apoptosis and migration in endothelial progenitor cells, promotes capillary-like tube formation through regulation of Bcl-2, Akt, NF-κB, p38MAPK and JNK expressions[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

α-Tocopherol phosphate Related Antibodies

Western Blot Analysis[2]

Cell Line: Endothelial progenitor cells derived from rat bone marrow
Concentration: 100 μM
Incubation Time: 24 h
Result: Upregulated the expression of Bcl-2 and Akt.
Downregulated the expression of Tie2, Notch1, p38MAPK and JNK.

Cell Migration Assay [2]

Cell Line: Endothelial progenitor cells derived from rat bone marrow
Concentration: 100 μM
Incubation Time: 24 h
Result: Promoted the cell migration.
In Vivo

α-Tocopherol phosphate (100 μM, intramuscular, single dose) exhibits angiogenesis-promoting activity in Streptozotocin (HY-13753) and ligation induced rats type 2 diabetes and ischemia models[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Rats type 2 diabetes and ischemia models[2]
Dosage: 100 μM α-Tocopherol phosphate incubated with EPCs
Administration: 24 incubation, intramuscular injection of EPC into rats hind limbs
Result: Increased the capillary density of the ischemic hindlimbs.
Molecular Weight

510.69

Formula

C29H51O5P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1=C(O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CC2)C2=C(C)C(OP(O)(O)=O)=C1C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (195.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9581 mL 9.7907 mL 19.5814 mL
5 mM 0.3916 mL 1.9581 mL 3.9163 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.90 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Purity & Documentation
References

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