TRITC-conjugated anti-chicken primary antibodies are affinity-purified antibodies directed against chicken (Gallus gallus) immunoglobulins or specific chicken proteins and covalently labeled with tetramethylrhodamine isothiocyanate (TRITC). These direct-label reagents enable one-step fluorescent detection in microscopy and flow cytometry workflows requiring a red-orange emission channel. TRITC provides good brightness and can serve as a cost-effective alternative to modern red dyes for applications where extreme photostability is not critical.
Key Features
- Direct Fluorescent Labeling: Primary antibodies directly conjugated to TRITC for simplified one-step staining.
- Affinity Purified: Monoclonal and polyclonal formats are available to minimize nonspecific binding.
- Optimized D/P Ratio: Controlled dye-to-protein (D/P) ratio to balance fluorescence intensity while preserving antigen-binding activity.
- Flexible Formats: Supplied as liquid formulations (ready-to-use or concentrated) and lyophilized preparations. Azide-free formulations are available upon request for live-cell applications.
- Stabilized Formulations: Buffers commonly contain protein stabilizers such as BSA and preservatives including sodium azide, where indicated.
- Lot Quality Control: Each lot is tested for D/P ratio, purity by SDS-PAGE, and application-specific validation including immunofluorescence (IF) and flow cytometry (FACS).
Common Applications
- Immunofluorescence (IF) and Immunocytochemistry (ICC): Direct visualization of target antigens in fixed cells and tissue sections using standard TRITC filter sets.
- Confocal and Widefield Fluorescence Microscopy: Suitable for colocalization studies when combined with spectrally distinct fluorophores such as FITC, Alexa Fluor™ 488, or DAPI.
- Flow Cytometry (FACS): Compatible with single-color and multicolor panels utilizing a red-orange fluorescence channel and moderate brightness requirements.
- Plate-Based Fluorescent Assays: Suitable for fluorescence microplate readers configured with TRITC excitation and emission settings.
- Live-Cell Surface Staining: Applicable in selected cases when validated antibodies and azide-free formulations are used. Optimization and validation are recommended for each cell type.