Streptavidin-conjugated anti-chicken primary antibodies are affinity-purified antibodies directed against chicken (Gallus gallus) immunoglobulins or specific chicken proteins that are covalently linked to streptavidin. These reagents combine the antigen specificity of primary antibodies with the modular detection versatility of streptavidin, enabling direct capture of biotinylated targets or streamlined detection when used in combination with biotinylated secondary reagents, biotinylated probes, or biotinylated antigens. Streptavidin conjugation supports a wide range of detection modalities by pairing with biotinylated enzymes, fluorophores, or solid supports (beads, plates).
Key features
- Streptavidin conjugation: Covalent attachment of streptavidin to the antibody enables high-affinity binding to biotinylated partners.
- High purity: Affinity-purified monoclonal and polyclonal formats designed to reduce nonspecific binding.
- Versatile detection: Compatible with biotinylated substrates, probes, enzymes, fluorophores, and streptavidin bead systems for capture applications.
- Formats: Supplied in liquid or lyophilized form with stabilizers; azide-free options available on request.
- QC testing: Lot-specific validation for streptavidin activity, antibody binding, and performance in ELISA, Western blot, FACS, and IP.
Common applications
- Capture and pull-down assays: Enables immobilization of biotinylated antigens or probes on plates or beads for immunoprecipitation workflows.
- Sandwich ELISA: Used in capture or detection steps in combination with biotinylated reagents for flexible assay design.
- Western blot and dot blot: Detection of biotinylated proteins or use as a capture reagent on biotinylated membranes.
- Flow cytometry (FACS): Supports detection or capture of biotinylated ligands and bead-based assay systems.
- Immunoprecipitation and affinity purification: Enables efficient capture using biotin–streptavidin interactions for downstream analysis.
- Assay development: Allows modular switching of detection systems via different biotinylated conjugates.