Rhodamine-conjugated anti-monkey primary antibodies are fluorescent immunoreagents that employ rhodamine-class dyes as reporter molecules for the detection of non-human primate (NHP) antigens. Rhodamines constitute a family of xanthene-based fluorophores characterized by high extinction coefficients, good photostability, and low pH sensitivity, making them valuable alternatives to fluorescein-based conjugates in immunofluorescence microscopy, flow cytometry, and fluorescence-based immunoassays. When covalently linked to anti-monkey antibodies, rhodamine dyes enable sensitive and reproducible detection of cell surface and intracellular antigens in NHP models, including cynomolgus macaque, rhesus macaque, and baboon.
Anti-Monkey Antibody Targets and Applications
These conjugates are validated for multiple applications:
Flow cytometry and FACS: TRITC-conjugated anti-monkey antibodies are used for immunophenotyping of NHP leukocyte subsets, with typical working dilutions ranging from 1:500 to 1:2,500.
Immunofluorescence microscopy: Rhodamine conjugates enable sensitive detection of monkey antigens in fixed cells and tissue sections, with working dilutions typically ranging from 1:1,000 to 1:5,000.
Immunohistochemistry: Both frozen and paraffin-embedded tissue sections can be stained using rhodamine conjugates.
Western blotting and fluorescent ELISA (FLISA): Rhodamine conjugates are compatible with membrane-based detection systems and plate-based assays.
Fab and F(ab')₂ fragment conjugates are also available for applications requiring reduced Fc receptor binding and lower background signal.