Mouse antibody arrays are multiplex protein analysis tools designed to quantify multiple mouse proteins simultaneously in biological samples. These antibody microarrays contain capture antibodies immobilized at defined positions on functionalized glass slides. In sandwich immunoassay formats, target proteins are captured by specific antibodies and detected using compatible detection antibodies and signal-generating reagents. For fluorescence-based arrays, signals are measured using a microarray scanner. When appropriate calibration standards and validated standard curves are available, signal intensities can be used to estimate analyte concentrations. Quantitative mouse antibody arrays support the investigation of cytokines, chemokines, growth factors, soluble receptors, and other signaling molecules involved in immune responses and disease mechanisms.
Key Features
-
Multiplex protein quantification: Simultaneous measurement of multiple selected mouse proteins in a single sample.
-
Glass slide format: Spatially arranged capture antibodies enable parallel detection of multiple analytes.
-
Quantitative readout: Validated calibration curves support concentration estimation within the assay's working range.
-
Sample compatibility: Depending on assay validation, suitable samples may include serum, plasma, cell culture supernatants, and tissue or cell lysates.
Usual Applications
-
Profiling cytokines and chemokines in mouse models of inflammation and immune responses.
-
Comparing protein levels across experimental conditions or treatment groups.
-
Investigating tumor microenvironments, cell signaling, and disease-associated protein changes.
-
Screening candidate protein biomarkers for subsequent validation.
Quantitative results depend on antibody specificity, sample handling, calibration, and the validated measuring range of each analyte.
