Human Obesity Antibody Arrays on Glass Slides are multiplex protein profiling tools designed for the simultaneous detection and semi-quantitative analysis of obesity-related proteins in human biological samples. Based on antibody microarray technology, they use specific capture antibodies immobilized at predefined positions on functionalized glass slides to detect adipokines, metabolic regulators and inflammatory mediators. Following sample incubation, bound proteins are detected using specific detection antibodies and fluorescent labeling, with signal intensities measured by fluorescence microarray scanning. These arrays support the investigation of adipose tissue biology, metabolic regulation and obesity-associated inflammation.
Key Features
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Multiplex detection: Simultaneous profiling of multiple obesity-related proteins, including leptin, adiponectin, resistin, chemerin, insulin and inflammatory cytokines.
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Glass-slide format: Spatially arranged capture antibodies enable parallel protein detection within a single assay.
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Semi-quantitative analysis: Relative protein levels are evaluated by comparing fluorescence signal intensities, with normalization according to the assay protocol.
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Human sample compatibility: Depending on array specifications, samples may include serum, plasma, cell culture supernatants and tissue lysates.
Usual Research Applications
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Investigating adipokine secretion and adipocyte function.
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Studying obesity-associated inflammation and metabolic dysregulation.
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Comparing protein expression profiles between lean and obese conditions.
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Exploring molecular pathways involved in insulin resistance and obesity-related metabolic disorders.
