Atto-conjugated anti-monkey primary antibodies are fluorescent immunoreagents that combine the photostability and brightness of ATTO-TEC dyes with the target specificity of immunoglobulins directed against non-human primate antigens. These reagents enable sensitive detection of monkey antigens in flow cytometry, immunofluorescence microscopy, and other fluorescence-based assays, making them indispensable in preclinical research, vaccine development, and translational medicine.
Applications
Flow Cytometry and FACS. The emission spectra of ATTO conjugates match the principal output wavelengths of most common fluorescence instrumentation, enabling their use on standard flow cytometers.
Immunofluorescence Microscopy. ATTO dyes are highly suitable for high-resolution microscopy techniques including PALM, dSTORM, and STED, as well as conventional fluorescence microscopy. The high photostability of ATTO dyes makes them particularly valuable for imaging applications requiring prolonged illumination.
Immunohistochemistry. ATTO-conjugated anti-monkey antibodies are validated for immunohistochemical staining of frozen and paraffin-embedded tissue sections. PE/Atto 594-conjugated anti-monkey IgG and IgM antibodies are validated for IHC-Fr, IHC-P, Western blot, and ELISA applications.
Western Blotting and ELISA. ATTO-conjugated anti-monkey antibodies are employed in Western blotting and ELISA for detection of monkey immunoglobulins and antigens, with working dilutions typically determined empirically for each application.
