AP-conjugated anti-Drosophila primary antibodies are affinity-purified antibodies raised against Drosophila melanogaster proteins or immunoglobulins and directly labeled with alkaline phosphatase (AP). These one-step reagents eliminate secondary antibodies, reducing assay time and minimizing background while providing robust chromogenic and chemifluorescent detection across multiple applications.
Key Features
- Direct AP conjugation: Enables rapid single-step detection with reduced cross-reactivity.
- High purity: Affinity-purified monoclonal and polyclonal antibodies ensure excellent specificity.
- Optimized performance: Controlled conjugation preserves antigen binding and AP activity.
- Versatile substrates: Compatible with NBT/BCIP, BCIP/NBT, AttoPhos, CDP-Star, and other chromogenic or fluorescent systems.
Applications
Ideal for western blotting, immunohistochemistry, in situ hybridization, ELISA, dot blotting, tissue blotting, and multiplex chromogenic workflows. AP-generated signals are stable, highly sensitive, and well suited for quantitative analysis, long-term slide archiving, and routine laboratory research.