Alexa Fluor®-conjugated anti-mouse primary antibodies are fluorescent immunoreagents that combine the exceptional brightness and photostability of the Alexa Fluor dye family with the target specificity of immunoglobulins directed against mouse antigens. Because the laboratory mouse (Mus musculus) is the most widely used mammalian model organism in biomedical research, reagents that enable sensitive and reproducible detection of mouse proteins are indispensable. The Alexa Fluor family is available across a broad spectral range and is compatible with common excitation sources, enabling flexible integration into existing instrument configurations.
Applications
Alexa Fluor-conjugated anti-mouse antibodies are validated across a broad spectrum of immunoassay applications:
- Flow cytometry: Immunophenotyping of mouse leukocyte subsets, stem cell markers, and activation markers, with working dilutions typically ranging from 0.5 µg per test to 1:500 depending on the product.
- Immunofluorescence microscopy: Localization of mouse antigens in cultured cells and tissue sections, with working dilutions typically ranging from 1:200 to 1:2,000.
- Immunohistochemistry: Staining of frozen and formalin-fixed, paraffin-embedded (FFPE) mouse tissue sections, with recommended concentrations of 1–10 µg/mL for most applications.
- Western blotting: Detection of mouse proteins using fluorescent or chemiluminescent detection, with working dilutions typically ranging from 1:2,500 to 1:5,000 for secondary antibodies.
