Phycoerythrin (PE)/Atto 594-conjugated anti-pig primary antibodies are tandem fluorochrome reagents that enable direct, single-step detection of porcine antigens in multicolor flow cytometry. They are particularly valuable in swine immunology, where the reagent landscape is less mature than in human or mouse systems.

Introduction

PE/Atto 594 is a tandem fluorochrome in which R-phycoerythrin (R-PE) serves as the 488 nm-excited donor and the rhodamine-class dye Atto 594 functions as the acceptor. The tandem exhibits an excitation peak at 565 nm (with efficient 488 nm excitation via the PE donor) and an emission peak at 627 nm. This spectral profile positions it as a direct alternative to PE-Dazzle 594, PE-Alexa 610, PE-eFluor 610, and PE-CF594. As a directly labeled primary antibody, it eliminates the need for a secondary reagent, reducing assay time and avoiding the cross-reactivity that can arise when anti-pig secondary reagents detect endogenous porcine immunoglobulins.

Applications

The PE/Atto 594 format is validated primarily for flow cytometry of porcine samples, with additional support for immunofluorescence, immunohistochemistry, immunocytochemistry, and Western blotting depending on the clone. Its 627 nm emission is well separated from the green FITC channel (~519 nm) and the far-red APC channel (~660 nm), making it well suited to multicolor panel design alongside these common fluorophores. A documented application is the PE/Atto 594 CD3ε conjugate (clone PPT3) for flow cytometric identification of porcine T lymphocytes. Catalog listings indicate that the PE/Atto 594 format is available across a range of anti-pig specificities, with pig reactivity represented among the 35 products in this conjugate class.