APC conjugated anti-cat primary antibodies are fluorescent immunoreagents used to detect feline antigens while providing a strong signal in the far-red emission range. They are commonly used in flow cytometry and fluorescence-based assays where bright, low-background detection is required. Their direct labeling with APC simplifies experimental workflows by eliminating the need for a secondary antibody.
APC Fluorophore Properties
Allophycocyanin (APC) is a highly fluorescent phycobiliprotein characterized by strong brightness and excellent performance in multicolor detection panels. It is efficiently excited by red lasers and emits in the far-red spectrum, reducing spectral overlap with commonly used fluorophores such as FITC, PE, or PerCP. This property makes APC conjugates particularly suitable for complex multiplex assays.
Antibody Specificity and Format
These antibodies are designed to recognize cat-specific proteins, including immune markers, structural proteins, and other feline antigens depending on the application. The APC fluorophore is covalently attached to the antibody in a manner that preserves antigen-binding activity. As primary antibodies, they bind directly to the target, enabling streamlined direct detection workflows.
Applications in Feline Research
APC conjugated anti-cat primary antibodies are widely used in flow cytometry, immunophenotyping, and fluorescent cell analysis. They support studies in feline immunology, infectious diseases, inflammatory conditions, and biomarker profiling. In fluorescence microscopy applications, they can also be used when direct labeling is preferred to reduce background and shorten experimental procedures.
