APC-conjugated anti-Drosophila primary antibodies are affinity-purified monoclonal or polyclonal antibodies directed against Drosophila melanogaster proteins or immunoglobulins and directly labeled with Allophycocyanin (APC). This bright far-red fluorophore is excited by 633–647 nm lasers and emits around 660–670 nm, providing excellent sensitivity with minimal cellular autofluorescence. Direct conjugation eliminates secondary antibodies, streamlining workflows while reducing assay time and background.
Key Features
- High fluorescence: APC delivers exceptional brightness for detecting low-abundance targets.
- Direct detection: One-step staining improves reproducibility and minimizes cross-reactivity.
- Quality controlled: Optimized dye-to-protein ratio, high purity, and functional validation ensure reliable performance.
Applications
These antibodies are widely used in multicolor flow cytometry (FACS), cell sorting, bead-based assays, and far-red fluorescence microscopy. Their intense APC signal is particularly valuable for immunophenotyping, rare-cell analysis, and imaging applications requiring high sensitivity with reduced autofluorescence.