Y-27632 is a ROCK inhibitor with K_i values of 220 nM and 300 nM for ROCK1 and ROCK2, respectively. Y-27632 exerts anti-inflammatory and immunomodulatory effects in systemic lupus erythematosus models by inhibiting the ROCK/NF-κB pathway. Y-27632 enhances autophagy by inhibiting the AKT/mTOR pathway, thereby inducing apoptosis apoptosis in oral squamous cell carcinoma. Y-27632 induces the formation of tunneling nanotubes in ARPE-19 cells and significantly enhances mitochondrial transfer through these channels. Y-27632 promotes neurite outgrowth in PC12 cells by activating the Rac1/NOX1/ROS/AKT/PAK1 signaling cascade^{[1][2][3][4][5][6]}.

Y-27632 (10 μM; 24-72 h) significantly reduces the viability of CAL-27, SCC-4 and SCC-9 oral squamous cell carcinoma (OSCC) cells^[2].
Y-27632 (10 μM; 24 h) significantly inhibits the migration of CAL-27, SCC-4 and SCC-9 cells^[2].
Y-27632 (10 μM; 6-24 h) inactivates the AKT/mTOR pathway, reduces the levels of phosphorylated downstream effectors, and increases the levels of apoptosis markers in OSCC cells^[2].
Y-27632 (10 μM) induces autophagy in OSCC cells by inhibiting the mTOR pathway and promoting the conversion of LC3-I to LC3-II^[2].
Y-27632 (10-50 μM; 5 min-3 h) induces dose- and time-dependent neurite outgrowth in PC12 cells via the Rac1/NOX1/ROS/AKT/PAK1 signaling cascade^[4].
Y-27632 (10-1000 μM; 24 h) dose-dependently promotes the formation of F-actin/tubulin-containing Y-NTs (average length 30 μm) in ARPE19 retinal pigment epithelial cells and increases the mitochondrial transfer rate; this effect also exists under photodamage conditions, and mitochondrial transfer depends on direct contact between cells^[5].
Y-27632 (40 μM; 24 h) induces cytoskeleton remodeling and morphological changes (increased cell area, enhanced contour length and pseudopodium formation) in ARPE19 retinal pigment epithelial cells, without altering the protein expression of F-actin or α-tubulin^[5].
Y-27632 (40 μM; 24 h) alters mitochondrial distribution to an infiltrative and axon-like pattern, enhances mitochondrial mobility, upregulates miro1 mRNA expression, and increases the number and length of individual mitochondria in ARPE19 retinal pigment epithelial cells^[5].
Y-27632 (10-1000 μM; 24 h) reduces the viability and promotes the apoptosis of ARPE19 retinal pigment epithelial cells only when the concentration exceeds 40 μM, and exerts no significant effects at concentrations of 10 μM, 20 μM or 40 μM^[5].
Y-27632 (40 μM; 24 h) induces Y-shaped nanotubes (Y-NTs) in ARPE19 retinal pigment epithelial cells, a process that depends primarily on F-actin; in contrast, Y-27632-enhanced mitochondrial transport via Y-NTs relies on both F-actin and microtubules^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Wang Y, et al. Y-27632, a Rho-associated protein kinase inhibitor, inhibits systemic lupus erythematosus. Biomed Pharmacother. 2017;88:359-366.

  [2]. Wen J, et al. Y-27632 Suppresses the Growth and Migration of Oral Squamous Cell Carcinoma, but Upregulates Autophagy by Suppressing mTOR Effectors. J Oral Pathol Med. 2025;54(4):207-216.  [Content Brief]

  [3]. Dong LY, et al. Y-27632, a Rho-kinase inhibitor, attenuates myocardial ischemia-reperfusion injury in rats. Int J Mol Med. 2019;43(4):1911-1919.  [Content Brief]

  [4]. Park SY, et al. Y-27632 Induces Neurite Outgrowth by Activating the NOX1-Mediated AKT and PAK1 Phosphorylation Cascades in PC12 Cells. Int J Mol Sci. 2020;21(20):7679. Published 2020 Oct 16.  [Content Brief]

  [5]. Yuan J, et al. ROCK inhibitor enhances mitochondrial transfer via tunneling nanotubes in retinal pigment epithelium. Theranostics. 2024;14(15):5762-5777. Published 2024 Sep 9.  [Content Brief]

  [6]. Ishizaki T, et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol. 2000 May;57(5):976-83.  [Content Brief]