Includes for 1,000 assays:
– 2 x 25 mL XTT Cell Proliferation Assay Kit Reagent
– 1 mL Activation Reagent

• Cell proliferation and viability quantification.

• Drug screening and cytotoxicity testing.

• Toxicology assays with chemicals and nutrients.

• Environmental compound effect analysis.

• High-throughput screening in microplates.

• Lot-to-lot reproducibility ensured.

• Tested for sensitivity and linearity in cell-based assays.

• Protect reagents from light.

• Use freshly prepared activation reagent.

• Include positive and negative controls for accuracy.

• Shipped at: Gel Pack.
• Storage: -20 °C.
• Recommendations: Protect XTT solution from light.

• Carletti, A., Pes, K., Tarasco, M., Rosa, J. T., Poudel, S., Pereira, H., … & Gavaia, P. Immunomodulatory inhibition of osteoclastogenesis by a marine microalgal ethanol fraction targeting T-cells, antigen presentation, and macrophage fate. Frontiers in Immunology, 16, 1655321.
• Usala, E., Gonzalez, Z., Campillo, N., Baena, J., Ferrari, B., Rodríguez, A., & Espinosa, E. (2025). Impact of Steam Sterilization on the Rheological Characteristics, Printability, and Bioactivity of Nanocellulose/Alginate Hydrogels for 3D Bioprinting. Carbohydrate Polymer Technologies and Applications, 100970.
• Carletti, A., Pes, K., Tarasco, M., Rosa, J. T., Poudel, S., Pereira, H., … & Gavaia, P. J. (2025). Harnessing the immune system to treat bone loss: The immunomodulatory and osteoprotective effects of the microalga Skeletonema costatum. bioRxiv, 2025-02.
• Tavares Pereira, M., Kazemian, A., Rehrauer, H., & Kowalewski, M. P. (2022). Transcriptomic profiling of canine decidualization and effects of antigestagens on decidualized dog uterine stromal cells. Scientific Reports, 12(1), 21890.
• Kuczwara, V., Schuler, G., Pfarrer, C., Tiedje, L., Kazemian, A., Pereira, M. T., … & Klisch, K. (2023). Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition. Veterinary Sciences, 10(1), 44.
• Hervás-Corpión, I., Navarro-Calvo, J., Martín-Climent, P., Iriarte-Gahete, M., Geribaldi-Doldán, N., Castro, C., & Valor, L. M. (2023). Defining a Correlative Transcriptional Signature Associated with Bulk Histone H3 Acetylation Levels in Adult Glioblastomas. Cells, 12(3), 374.
• Zaballos, M. A., Acuña-Ruiz, A., Morante, M., Riesco-Eizaguirre, G., Crespo, P., & Santisteban, P. (2022). Inhibiting ERK dimerization ameliorates BRAF-driven anaplastic thyroid cancer. Cellular and Molecular Life Sciences, 79(9), 1-19.
• Ramírez-Moya, J., Wert-Lamas, L., Acuña-Ruíz, A., Fletcher, A., Wert-Carvajal, C., McCabe, C. J., … & Riesco-Eizaguirre, G. (2022). Identification of an interactome network between lncRNAs and miRNAs in thyroid cancer reveals SPTY2D1-AS1 as a new tumor suppressor. Scientific reports, 12(1), 1-13.
• Ramírez-Moya, Julia, et al. “An ADAR1-dependent RNA editing event in the cyclin-dependent kinase CDK13 promotes thyroid cancer hallmarks.” Molecular Cancer 20.1 (2021): 1-20.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

XTT cell proliferation assay, Cell viability assay XTT, Tetrazolium cell proliferation assay, Metabolic activity assay XTT, Colorimetric cell viability assay

Also known as:

• Spanish: Kit de proliferación celular XTT, Ensayo de viabilidad celular XTT, Ensayo colorimétrico celular
• French: Kit de prolifération cellulaire XTT, Test de viabilité cellulaire XTT, Essai colorimétrique cellulaire
• German: XTT-Zellproliferationsassay, Zellviabilitätsassay XTT, Kolorimetrischer Zelltest
• Italian: Kit proliferazione cellulare XTT, Saggio vitalità cellulare XTT, Saggio colorimetrico cellulare

Q: What is the detection principle of the XTT assay?
A: Reduction of XTT to a soluble formazan dye by viable cells.

Q: How long does the assay take?
A: Results in 2–5 hours.

Q: Does it require solubilization of the dye?
A: No, formazan is water-soluble.

Q: What equipment is required for detection?
A: A microplate reader capable of measuring absorbance at 450 nm is required.