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> Tryptone [73049-73-7]

Tryptone [73049-73-7]

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Referência HY-W133898-25g

Tamanho : 25g

Marca : MedChemExpress

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Description

Tryptone is a peptide-rich bacterial culture medium component that can regulate bacterial cell surface proteins and biofilm-related genes. Tryptone promotes the expression and assembly of bacterial adhesion proteins (such as LapA and LapF) by providing peptide substances as structural factors, enhancing cell surface hydrophobicity and intercellular adhesion, thereby stabilizing the biofilm matrix and supporting the maturation and maintenance of bacterial biofilms. The peptide mixture contained in Tryptone can specifically regulate the transcription of bacterial adhesion-related genes (such as activating LapA and inhibiting LapF), affecting the synthesis and localization of biofilm structural proteins[1][2].

In Vitro

Tryptone (10 g/L; 10 min; 37°C) significantly improved the capture efficiency of T4 phage conjugated magnetic particles for Escherichia coli, and this effect was temperature-dependent[1].
In the Pseudomonas biofilm formation experiment, Tryptone (10 g/L; 24 h) promoted the formation of mature biofilms of wild-type and Fis-overexpressing strains of Pseudomonas putida, increasing the amount of biofilm by 1.3-2.9 times compared with the M9 medium group without Tryptone, and was related to peptide-mediated cell surface protein regulation[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tryptone Related Antibodies

Cell Viability Assay[1]

Cell Line: Escherichia coli (ATCC 11303)
Concentration: 10 g/L (added to Luria-Bertani medium or phosphate buffer)
Incubation Time: 10 min at 37°C
Result: Increased the capture efficiency of T4 bacteriophage-conjugated Fe3O4 magnetic particles, with viable bacterial counts in the supernatant decreasing by 70% compared to Tryptone-free conditions.
Bacterial capture was temperature-dependent, with no significant effect observed at 4°C. Flow cytometry and plate counting assays showed that Tryptone facilitated irreversible binding between T4 bacteriophage and E. coli surface receptors, enhancing magnetic particle-mediated isolation of bacteria.

Cell Viability Assay[2]

Cell Line: Pseudomonas putida strains PSm (wild type) and F15 (fis-overexpression)
Concentration: 10 g/L (supplemented to M9-0.2CAA medium)
Incubation Time: 24 h
Result: Increased biofilm formation measured by crystal violet staining, with wild-type PSm showing a 1.3-fold increase and F15 strain exhibiting a 2.9-fold increase compared to Tryptone-free M9 medium.
qPCR analysis revealed upregulated expression of adhesion genes lapA and downregulated lapF in F15 cells, while Western blot confirmed enhanced Fis protein levels in Tryptone-supplemented cultures.
Biofilm matrix stability was assessed via protease K treatment, showing reduced dispersion in Tryptone-containing media, indicating polypeptide-mediated reinforcement of extracellular matrix structure.
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

[Tryptone]
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

H2O : 100 mg/mL (Need ultrasonic)

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