Tamanho : 20×100ul
Telefone : +1 850 650 7790
Trans10 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to StrR.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15Δ lacX74 recA1 araΔ139Δ(ara-leu)7697 galU galKrpsL (StrR) endA1 nupG
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• StrR.
• Blue/white selection.
• Toxic gene cloning and stable replication of plasmid DNA.
Storage
at -70℃ or below for one year. Do not store in liquid nitrogen.
Shipping
Dry ice (-70℃)
1 Yao H, Song Y, Chen Y, et al. Molecular architecture of the SARS-CoV-2 virus[J]. Cell, 2020.(IF 66.85)
2 Wang X, Xuan Y, Han Y, et al. Regulation of HIV-1 Gag-Pol expression by shiftless, an inhibitor of programmed-1 ribosomal frameshifting[J]. Cell, 2019.(IF 64.50)
3 Zhong S, Zhang S, Fan X, et al. A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex[J]. Nature, 2018.(IF 40.13)
Manual