Biological Activity

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM. Torin 1 shows over 100-fold selectivity for mTOR over PI3Kα and other PI3K-like kinases, including ATM, DNA-PK, and hVps34, as well as 450 other protein kinases. Torin 1 induces autophagy in HeLa cells. Torin 1 is efficacious at a dose of 20 mg/kg in inhibiting downstream effectors of mTOR in mice. In a recent study, it is reported Torin 1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON.

Product Citations

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  Commun Biol. 2023 Apr 3;6(1):361.

  WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis
  Torin 1 purchased from AbMole

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  Cell Cycle. 2022 Sep 19;1-23.

  Mechanistic target of rapamycin (mTOR) regulates self-sustained quiescence, tumor indolence and late clinical metastasis in a Beclin-1-dependent manner.
  Torin 1 purchased from AbMole

Chemical Information

Molecular Weight	607.62
Formula	C35H28F3N5O2
CAS Number	1222998-36-8
Solubility (25°C)	DMSO ~2 mg/mL (ultrasonic)
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Peterson TR, et al. Cell. mTOR complex 1 regulates lipin 1 localization to control the SREBP pathway.

[2] Li J, et al. Am J Physiol Cell Physiol. mTORC1 inhibition increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON.

[3] Dowling RJ, et al. Science. mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs.

[4] Thoreen CC, et al. J Biol Chem. An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1.