TH Antibody
Referência AO2215a
Tamanho : 100ul
Marca : Abcepta
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TH Antibody
Purified Mouse Monoclonal Antibody
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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Figure 2:Western blot analysis using TH mAb against human TH (AA: 44-208) recombinant protein. (Expected MW is 44 kDa)
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Figure 3:Western blot analysis using TH mAb against HEK293 (1) and TH (AA: 44-208)-hIgGFc transfected HEK293 (2) cell lysate.
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Figure 4:Western blot analysis using TH mouse mAb against SH-SY5Y (1) and PC-12 (2) cell lysate.
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Figure 5:Immunofluorescence analysis of Hela cells using TH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Figure 6:Flow cytometric analysis of Hela cells using TH mouse mAb (green) and negative control (red).
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Figure 7:Flow cytometric analysis of MCF-7 cells using TH mouse mAb (green) and negative control (red).
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Figure 8:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TH mouse mAb with DAB staining.
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Application 
| WB, IHC, FC, ICC, E |
|---|---|
| Primary Accession | P07101 |
| Reactivity | Human, Rat |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1B8D2 |
| Isotype | IgG1 |
| Calculated MW | 58.6kDa |
| Description | The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. |
| Immunogen | Purified recombinant fragment of human TH (AA: 44-208) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Gene ID | 7054 |
|---|---|
| Other Names | Tyrosine 3-monooxygenase, 1.14.16.2, Tyrosine 3-hydroxylase, TH, TH, TYH |
| Dilution | WB~~1/500 - 1/2000 IHC~~1/200 - 1/1000 FC~~1/200 - 1/400 ICC~~N/A E~~1/10000 |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TH Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TH (HGNC:11782) |
|---|---|
| Synonyms | TYH |
| Function | Catalyzes the conversion of L-tyrosine to L- dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of catecholamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed:15287903, PubMed:1680128, PubMed:17391063, PubMed:24753243, PubMed:34922205, PubMed:8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity). |
| Cellular Location | Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P24529}. Nucleus {ECO:0000250|UniProtKB:P04177} Cell projection, axon {ECO:0000250|UniProtKB:P24529}. Cytoplasm {ECO:0000250|UniProtKB:P04177}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle {ECO:0000250|UniProtKB:P04177}. Note=When phosphorylated at Ser-19 shows a nuclear distribution and when phosphorylated at Ser-31 as well at Ser-40 shows a cytosolic distribution (By similarity). Expressed in dopaminergic axons and axon terminals. {ECO:0000250|UniProtKB:P04177} |
| Tissue Location | Mainly expressed in the brain and adrenal glands. |
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