TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (AP)
Referência MBS6123688-5x0,1mL
Tamanho : 5x0.1mL
Marca : MyBiosource
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TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (AP)
TGF-alpha Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6123688MBS6123688 is an antibody targeting TGF-alpha. Also known as TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (AP). Reactive in Human, Rabbit, Zebrafish. Host: Mouse. More details
Product Overview
Online Datasheet in PDFProduct Name
TGF-alpha, Monoclonal Antibody
Full Product Name
TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (AP)
Product Gene Name
anti-TGF-alpha antibody
Product Synonym Names
EGF-like TGF; ETGF; TFGA; TGF Type 1; TGFA; Wa1; Waved 1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E8-G6
Host
Mouse
Species Reactivity
Human, Rabbit, Zebrafish
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human TGF-alpha. Species Crossreactivity: rabbit, and zebrafish TGF-alpha. Others-not known.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Molecular Weight
17,006 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (70674412..70781147, complement). Location: 2p13
OMIM
190170
Immunogen
A 10-amino acid synthetic peptide (aa34-43) from human TGFalpha. Cellular Localization: Cytoplasmic and secreted
Conjugate
AP
Positive Control
Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma
Product Categories/Family for anti-TGF-alpha antibody
Antibodies; Growth Factors; Cytokines
Applications Tested/Suitable for anti-TGF-alpha antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP)
Application Notes for anti-TGF-alpha antibody
IHC-F/P: 1-2ug/ml for 30 min at RT
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
Applications are based on unconjugated antibody.
IP: 1-2ug/500ug protein
IF: 1-2ug/ml
Applications are based on unconjugated antibody.
Product References and Citations for anti-TGF-alpha antibody
1. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993, 134(24):1303-7. Language: Hungarian. 2. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35. 3. Nasim, M. M., Thomas, D. M., Alison, M. R., and Fil]ipe, M. I. Transforming growth factor a expression in normal gastric mucosa, intestinal metaplasia, dysplasia and gastric carcinoma - an immunohistochemical study, Histopathology. 20: 339-343, 1992.
Other Notes
Small volumes of anti-TGF-alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for TGF-alpha. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor, alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
UniProt Protein Name
Protransforming growth factor alpha
UniProt Synonym Protein Names
EGF-like TGF; ETGF; TGF type 1
Protein Family
Transforming growth factor
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
UniProt Entry Name
TGFA_HUMAN
NCBI Protein Information
protransforming growth factor alpha; TGF-alpha
NCBI Summary for TGF-alpha
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Show more UniProt Comments for TGF-alpha
TGFalpha: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2. Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, integral; Cell cycle regulation
Chromosomal Location of Human Ortholog: 2p13
Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus
Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; response to drug; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Show more Protein type: Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, integral; Cell cycle regulation
Chromosomal Location of Human Ortholog: 2p13
Cellular Component: extracellular space; cell surface; integral to plasma membrane; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; cytoplasmic vesicle; nucleus
Molecular Function: protein binding; growth factor activity; glycoprotein binding; epidermal growth factor receptor binding
Biological Process: epidermal growth factor receptor signaling pathway; response to drug; cell proliferation; wound healing; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; angiogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-TGF-alpha antibody
| Reference Product | PubMed Publications |
|---|---|
| EGFR antibody | >34 publications with TGF-alpha and EGFR |
| ERBB2 antibody | >7 publications with TGF-alpha and ERBB2 |
| ERBB4 antibody | >3 publications with TGF-alpha and ERBB4 |
| ADAM17 antibody | >1 publications with TGF-alpha and ADAM17 |
Pathways associated with anti-TGF-alpha antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| Direct P53 Effectors Pathway antibodies | Direct P53 Effectors Pathway Diagram |
| ErbB Receptor Signaling Network Pathway antibodies | ErbB Receptor Signaling Network Pathway Diagram |
| ErbB Signaling Pathway antibodies | ErbB Signaling Pathway Diagram |
| ErbB Signaling Pathway antibodies | ErbB Signaling Pathway Diagram |
| ErbB Signaling Pathway antibodies | ErbB Signaling Pathway Diagram |
| FOXM1 Transcription Factor Network Pathway antibodies | FOXM1 Transcription Factor Network Pathway Diagram |
| Glioma Pathway antibodies | Glioma Pathway Diagram |
| Glioma Pathway antibodies | Glioma Pathway Diagram |
| Non-small Cell Lung Cancer Pathway antibodies | Non-small Cell Lung Cancer Pathway Diagram |
| Non-small Cell Lung Cancer Pathway antibodies | Non-small Cell Lung Cancer Pathway Diagram |
Diseases associated with anti-TGF-alpha antibody
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms Antibodies | >59 publications with TGF-alpha and Neoplasms |
| Genetic Predisposition to Disease Antibodies | >16 publications with TGF-alpha and Genetic Predisposition to Disease |
| Fatty Liver Antibodies | >16 publications with TGF-alpha and Fatty Liver |
| Liver Diseases Antibodies | >10 publications with TGF-alpha and Liver Diseases |
| Liver Neoplasms Antibodies | >10 publications with TGF-alpha and Liver Neoplasms |
| Head and Neck Neoplasms Antibodies | >9 publications with TGF-alpha and Head and Neck Neoplasms |
| Breast Neoplasms Antibodies | >9 publications with TGF-alpha and Breast Neoplasms |
| Neoplasms, Experimental Antibodies | >7 publications with TGF-alpha and Neoplasms, Experimental |
| Nervous System Diseases Antibodies | >7 publications with TGF-alpha and Nervous System Diseases |
| Carcinoma, Hepatocellular Antibodies | >7 publications with TGF-alpha and Carcinoma, Hepatocellular |
Organs/Tissues associated with anti-TGF-alpha antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >4 publications with TGF-alpha and Brain |