Product Details

SuperG™ Blocking Buffer – 500mL

SuperG™ Blocking Buffer optimizes the use of nitrocellulose for protein assays using fluorescence detection. Results with SuperG™ show significant reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Purified, protein-free solution has high binding efficiency for rapid and comprehensive blocking of non-specific protein.

 

SuperNOVA Film-Slides were assayed for IL1-a, IL1-b, IL6, TNF, and INF in a multiplexed antibody capture assay and detected with TRITC- or Alexa647-labeled secondary antibodies. Data are the background fluorescent intensities (Mean ± S.D.) for N=4 slides.

 

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Trial sizes may be available for purchase, for more information contact a Scientist

Technical Resources

Instructions

Instructions for Use - SuperG Blocking Buffer

Safety Data Sheet

SuperG Blocking Buffer

Applications

  • RPPA- reverse phase protein microarrays
  • Laser microdissection- RRPA
  • Antigen-capture assay
  • Antibody capture assay
  • Biomarker Discovery and Validation
  • Nanotoxicology
  • Serological Assay
  • Immunogen Discovery

References

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FAQ

Q Which buffer should Super G blocking buffer be diluted in and which dilution should be used?

A Super G  blocking buffer may be diluted with PBS.  0.5 to 0.1 x is the recommended dilution range.