Sulfo-NHS-SS-biotin sodium is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin sodium can be used for the labeling and purifying of cell-surface protein^[1].

IC50: cell-surface protein^[1]

Sulfo-NHS-SS-Biotin sodium is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes^[1].
Sulfo-NHS-SS-Biotin sodium reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides^[1].
Sulfo-NHS-SS-Biotin sodium has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns^[1].
Sulfo-NHS-SS-Biotin sodium (1 mg/mL; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS).
The biotinylation reactions are terminated with 100 mM glycine in PBS.
After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010).
Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Chia CP,et al. Phagocytosis in Dictyostelium discoideum is inhibited by antibodies directed primarily against common carbohydrate epitopes of a major cell-surface plasma membrane glycoprotein.Exp Cell Res. 1989 Mar;181(1):11-26.  [Content Brief]

  [2]. Jo M, et al. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 1;70(21):8948-58.  [Content Brief]