Stemolecule™ Y27632
04-0012 / 04-0012-10 / 04-0012-02
Brand: Stemolecule™
Y27632 is an inhibitor of Rho-associated kinase (ROCK), which is widely used to enhance the survival of dissociated PSCs. It is common to supplement the cell culture medium during the first 24 hours with 10 µM of Y27632 during cell passage or the establishment of spheroids.
CAS Number: 146986-50-7
Alternate Name(s):
- Y-27632
- (1R,4R)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
- ROCK Inhibitor
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Product Information
Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor1. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors3. Y27632 in combination with Pifithrin-u significantly improves cell recovery after cryopreservation4.
Stemolecule Y27632 is also available as a 10 mM DMSO Stock Solution in DMSO (2 mg in 624.4 μl) (Cat. No. 04-0012-02).
Y27632 is widely used in naive stem cell protocols5, such as the 5i/L/A/ protocol.
Product Name: Stemolecule Y27632
Catalog Number: 04-0012
Size:
- 2 mg (Cat. No. 04-0012)
- 10 mg (Cat. No. 04-0012-10)
- 2 mg solution in 10 mM DMSO (2 mg in 624.4 μl) (Cat. No. 04-0012-02)
Alternate Name(s):
- Y-27632
- (1R,4R)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
Chemical Formula: C14H21N3O·2HCl
Molecular Weight: 320.3
CAS Number: 146986-50-7
Purity: Greater than 97 % by HPLC analysis
Formulation: White solid
Solubility: Reconstitute in DMSO to the desired concentration. For reconstitution instructions please reference product specifications sheet.
Storage and Stability: Store powder at 4 °C protected from light. Following reconstitution, store aliquots at −20 °C. Stock solutions are stable for 6 months when stored as directed.
Quality Control: The purity of Y27632 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Y27632 was tested on mouse embryonic stem cells.
Specification Sheets:
Safety Data Sheets:
- Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983 (2000).
- Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25: 681-686 (2007).
- Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86: 270-280 (2008).
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- Fonseca, SAS; Costas, RM; Pereira, LV. Searching for naive human pluripotent stem cells. World Journal of Stem Cells, 7(3), 649 (2015).
Additional Publications
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