SSC Buffer 20X (Biotechnology Grade)
Catalog Number: B2010086 (50 mL)
SSC Buffer 20X is a concentrated solution that works as a standard reagent used in molecular biology for nucleic acid hybridization and blot transfer procedures, such as Southern and Northern blotting. This ready-to-use liquid serves to control stringency during washing steps and is certified free of DNase, RNase, and protease activity.
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Product Description
4.8/5 - (9 votes)
SSC Buffer 20X (Biotechnology Grade)
| Catalog number: | B2010086 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume/Weight | 50 mL |
| pH | 7 |
| Supplied as | 20X concentrate (working concentration ranges between 0.1X and 20X depending on application) |
| Appearance | Clear solution |
| Applications | SSC Buffer (20X) is a highly reproducible buffer for nucleic acid hybridization techniques. Working concentrations can be prepared by diluting the provided concentrate with di-water to a final concentration of 0.1X to 20X (depending on the application). |
| Shelf-Life | 1 year from date of manufacture |
| Storage: | Keep at 2-8°C |
| Keywords: | SSC buffer, SSC buffer 20X concentrate, ssc buffer in situ hybridization, saline-sodium citrate buffer, saline-sodium citrate 20X, ssc buffer dna hybridization |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Goldau T, Murayama K, Brieke C, Asanuma H, Heckel A. Azobenzene C-Nucleosides for Photocontrolled Hybridization of DNA at Room Temperature. Chemistry. 2015 Dec 1;21(49):17870-6.2: Markegard CB, Gallivan CP, Cheng DD, Nguyen HD. Effects of Concentration and Temperature on DNA Hybridization by Two Closely Related Sequences via Large- Scale Coarse-Grained Simulations. J Phys Chem B. 2016 Aug 18;120(32):7795-806.3: do Nascimento C, Muller K, Sato S, Albuquerque Junior RF. Effect of sample storage time on detection of hybridization signals in Checkerboard DNA-DNA hybridization. Can J Microbiol. 2012 Apr;58(4):502-6. 4: Avarre JC, de Lajudie P, Béna G. Hybridization of genomic DNA to microarrays: a challenge for the analysis of environmental samples. J Microbiol Methods. 2007 May;69(2):242-8. 5: Cho JC, Tiedje JM. Bacterial species determination from DNA-DNA hybridization by using genome fragments and DNA microarrays. Appl Environ Microbiol. 2001 Aug;67(8):3677-82. 6: Su X, Wu YJ, Knoll W. Comparison of surface plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization. Biosens Bioelectron. 2005 Nov 15;21(5):719-26.7: Hu Y, Yang T, Li Q, Guan Q, Jiao K. Conjugated self-doped polyaniline-DNA hybrid as trigger for highly sensitive reagentless and electrochemical self- signal amplifying DNA hybridization sensing. Analyst. 2013 Feb 21;138(4):1067-74. |
| Related Products | Biochemical Buffers and Solutions |
Additional Information
| Weight | 2.6 oz |
|---|---|
| Dimensions | 3.3 × 1.2 × 1.2 in |