Spironolactone [52-01-7]
Referência HY-B0561-1g
Tamanho : 1g
Marca : MedChemExpress
Spironolactone (SC9420) is an orally active aldosterone mineralocorticoid receptor antagonist with an IC50 of 24 nM. Spironolactone is also a potent antagonist of androgen receptor with an IC50 of 77 nM. Spironolactone promotes autophagy in podocytes.
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Spironolactone Chemical Structure
CAS No. : 52-01-7
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
Other Forms of Spironolactone:
- Spironolactone-d7 In-stock
- Spironolactone-d3 In-stock
- Spironolactone (Standard) Obtenir un devis
- Spironolactone-d3-1 Obtenir un devis
Description |
Spironolactone (SC9420) is an orally active aldosterone mineralocorticoid receptor antagonist with an IC50 of 24 nM. Spironolactone is also a potent antagonist of androgen receptor with an IC50 of 77 nM. Spironolactone promotes autophagy in podocytes[1][2][3]. |
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Essai clinique |
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Masse moléculaire |
416.57 |
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Formule |
C24H32O4S |
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CAS No. |
52-01-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
C[C@@]12[C@](OC3=O)(CC3)CC[C@@]1(04)[C@@]([C@@H](CC4=CC5=O)SC(C)=O)(04)[C@]([C@]4(CC5)C)(04)CC2 |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
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Solvant et solubilité |
In Vitro:
DMSO : ≥ 50 mg/mL (120.03 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation | |||||||||||||
Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.4006 mL | 12.0028 mL | 24.0056 mL | 60.0139 mL |
5 mM | 0.4801 mL | 2.4006 mL | 4.8011 mL | 12.0028 mL | |
10 mM | 0.2401 mL | 1.2003 mL | 2.4006 mL | 6.0014 mL | |
15 mM | 0.1600 mL | 0.8002 mL | 1.6004 mL | 4.0009 mL | |
20 mM | 0.1200 mL | 0.6001 mL | 1.2003 mL | 3.0007 mL | |
25 mM | 0.0960 mL | 0.4801 mL | 0.9602 mL | 2.4006 mL | |
30 mM | 0.0800 mL | 0.4001 mL | 0.8002 mL | 2.0005 mL | |
40 mM | 0.0600 mL | 0.3001 mL | 0.6001 mL | 1.5003 mL | |
50 mM | 0.0480 mL | 0.2401 mL | 0.4801 mL | 1.2003 mL | |
60 mM | 0.0400 mL | 0.2000 mL | 0.4001 mL | 1.0002 mL | |
80 mM | 0.0300 mL | 0.1500 mL | 0.3001 mL | 0.7502 mL | |
100 mM | 0.0240 mL | 0.1200 mL | 0.2401 mL | 0.6001 mL |