Specifications

Product Data
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	TrueORF Clone RC201828
Tag	C-Myc/DDK
Detection Antibodies	Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN	NM_005359, NP_005350
Synonyms	DPC4; JIP; MADH4; MYHRS
Predicted MW	60.3 kDa
Components	1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage	The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping	Blue Ice
Preparation	HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method	The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq	NP_005350
Locus ID	4089
Cytogenetics	18q21.2
Protein Families	WB
Protein Pathways	Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway

Product Manuals

Western Blot Protocols

FAQs

Over-expression Cell Lysate

SDS

SDS for LC401648

Recombinant Protein Resources

Video tutorial: Why protein expression host matters Webinar Video: Overexpression lysate as positive controls for Western blot Product Overview Video: Overexpression lysates as positive controls for Western blot Video Tutorial: Knockout(KO) lysate and antibody specificity Product Brochure: Protein

SKU	Description	Size
PH301828	SMAD4 MS Standard C13 and N15-labeled recombinant protein (NP_005350)	10 ug
LY401648	Transient overexpression lysate of SMAD family member 4 (SMAD4) (as WB positive control)	100 ug
TP301828	Recombinant protein of human SMAD family member 4 (SMAD4), 20 µg	20 ug
TP762318	Purified recombinant protein of Human SMAD family member 4 (SMAD4), Ala152-Tyr322, with N-terminal His tag, expressed in E.coli, 50ug	50 ug
TP762677	Purified recombinant protein of Human SMAD family member 4 (SMAD4), 1Met-195Tyr, with N-terminal His tag, expressed in E.coli, 50ug	50 ug