Sheep Leptin(LEP) ELISA kit
Referência CSB-EL012870SH-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
leptin
Associated names
LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
Abbreviation
Uniprot No.
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 82-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 86-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
Recovery
| The recovery of sheep LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Sheep LEP ELISA Kit was designed for the quantitative measurement of Sheep LEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.
Citations
- F YILMAZ,Kocatepe Veterinary Journal,2023
- Qiongxian Yan.et al,Italian Journal of Animal Science ,2017
- Leptin Levels and Lipids Profile Determination in Different Sheep Breeds Comba A.et al,Pakistan Veterinary Journal,2016
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism,...
Subcellular Location
Secreted.
Protein Families
Leptin family
Database Links
UniGene: