Images
Western Blot
Application

Western Blot

Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate, Lane 2: 293F cell transfection pcDNA3.0-ORF6-His. Western blot analysis of SARS-CoV-2 ORF6 polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell transfection pcDNA3.0-ORF6-His, Lane 4: 293F cell lysate (negative control).

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against partial synthetic peptide of SARS-CoV-2 ORF6.SARS-CoV-2,Coronavirus,COVID-19,COVID19,Wuhan virus,Wuhanvirus,PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide corresponding to amino acids 21-61 of SARS-CoV-2 ORF6.

    Host

    Rabbit

    Reactivity

    SARS-CoV-2

    Specificity

    The antibody detects endogenous levels of SARS-CoV-2 ORF6.

    Form

    Liquid

    Purification

    Affinity purification

    Purity

    > 95% by SDS-PAGE

    Isotype

    IgG

    Recommend Usage

    ELISA
    Western Blot (1:500)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (0.02% sodium azide).

    Storage Instruction

    Store at 4°C for short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Enzyme-linked Immunoabsorbent Assay

    Western Blot

    Western blot analysis of His-tag polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 1: 293F cell lysate, Lane 2: 293F cell transfection pcDNA3.0-ORF6-His. Western blot analysis of SARS-CoV-2 ORF6 polyclonal antibody (1:500) and Goat Anti-Rabbit IgG (H&L) secondary antibody (HRP) (1:20000), Lane 3: 293F cell transfection pcDNA3.0-ORF6-His, Lane 4: 293F cell lysate (negative control).
  • Publication Reference
    • Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants.

      Ann-Kathrin Reuschl, Lucy G Thorne, Matthew V X Whelan, Roberta Ragazzini, Wilhelm Furnon, Vanessa M Cowton, Giuditta De Lorenzo, Dejan Mesner, Jane L E Turner, Giulia Dowgier, Nathasha Bogoda, Paola Bonfanti, Massimo Palmarini, Arvind H Patel, Clare Jolly, Greg J Towers.

      Nature Microbiology 2024 Feb; 9(2):451.

      Application：WB, Human, Calu-3 cell line.

    • SARS-CoV-2 variants evolve convergent strategies to remodel the host response.

      Mehdi Bouhaddou, Ann-Kathrin Reuschl, Benjamin J Polacco, Lucy G Thorne, Manisha R Ummadi, Chengjin Ye, Romel Rosales, Adrian Pelin, Jyoti Batra, Gwendolyn M Jang, Jiewei Xu, Jack M Moen, Alicia L Richards, Yuan Zhou, Bhavya Harjai, Erica Stevenson, Ajda Rojc, Roberta Ragazzini, Matthew V X Whelan, Wilhelm Furnon, Giuditta De Lorenzo, Vanessa Cowton, Abdullah M Syed, Alison Ciling, Noa Deutsch, Daniel Pirak, Giulia Dowgier, Dejan Mesner, Jane L Turner, Briana L McGovern, M Luis Rodriguez, Rocio

      Cell 2023 Oct; 186(21):4597-4614..

      Application：WB.

    • Nanoscopic Elucidation of Spontaneous Self-Assembly of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Open Reading Frame 6 (ORF6) Protein.

      Goro Nishide, Keesiang Lim, Maiki Tamura, Akiko Kobayashi, Qingci Zhao, Masaharu Hazawa, Toshio Ando, Noritaka Nishida, Richard W Wong.

      The Journal of Physical Chemistry Letters 2023 Sep; 14(38):8385.

      Application：SDS-PAGE, WB, Human, A549, PC-9 cells, Recombinant proteins.

    • Evolution of enhanced innate immune evasion by SARS-CoV-2.

      Lucy G Thorne, Mehdi Bouhaddou, Ann-Kathrin Reuschl, Lorena Zuliani-Alvarez, Benjamin J Polacco, Adrian Pelin, Jyoti Batra, Matthew V X Whelan, Myra Hosmillo, Andrea Fossati, Roberta Ragazzini, Irwin Jungreis, Manisha Ummadi, Ajda Rojc, Jane Turner, Marie L Bischof, Kirsten Obernier, Hannes Braberg, Margaret Soucheray, Alicia Richards, Kuei-Ho Chen, Bhavya Harjai, Danish Memon, Joseph Hiatt, Romel Rosales, Briana L McGovern, Aminu Jahun, Jacqueline M Fabius, Kris M White, Ian Goodfellow, Yasu Ta

      Nature 2022 Feb; 602(7897):487-495.

      Application：WB.

    • Sarbecovirus ORF6 proteins hamper induction of interferon signaling.

      Izumi Kimura, Yoriyuki Konno, Keiya Uriu, Kristina Hopfensperger, Daniel Sauter, So Nakagawa, Kei Sato.

      Cell Reports 2021 Mar; 34(13):108916.

      Application：WB-Tr, Human, HEK 293 cells.

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