SAA1 ELISA Kit (Dog)
Referência OKEH03972
Tamanho : 96Wells
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SAA1 ELISA Kit (Dog) (OKEH03972)
| Datasheets/Manuals | Printable datasheet for SAA1 ELISA Kit (Dog) (OKEH03972) |
|---|
| Predicted Species Reactivity | Canis lupus|Dog | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology SAA1 ELISA Kit (Dog) (OKEH03972) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SAA1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Component |
| ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 1.36 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Dog Serum amyloid A protein | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | LOC403585, LOC751814 |
|---|---|
| Gene Full Name | serum amyloid A1 |
| Alias Symbols | SAA, serum amyloid A protein, serum amyloid A1. |
| NCBI Gene Id | 403585|751814 |
| Protein Name | Serum amyloid A protein |
| Description of Target | Major acute phase reactant. Apolipoprotein of the HDL complex. |
| Uniprot ID | P19708 |
| Protein Accession # | NP_001300801.1 |
| Nucleotide Accession # | NM_001313872.1 |
| Protein Size (# AA) | 129 |
| Molecular Weight | 129 kDa |
