Result Data
Result Data
Detection of ROS in Hela cells. Hela cells were seeded overnight at 15000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated (1 mM H2O2 or 100 uM tert-butyl hydroperoxide) or untreated (control) for 30 min at 37°C. The ROS Deep Red stain solution (100 uL/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal was monitored at Ex/Em = 650/675 nm (cut off = 665 nm) with bottom read mode using FlexStation.
- Specifications
Product Description
ROS Activity Assay Kit is a quantitative fluorometric determination of mitochondrial ROS activity.
Suitable Sample
Cell Culture
Label
Fluorometric
Detection Method
650/675 nm
Assay Type
Quantitative
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit at -20°C. For best results, please store the components as described in the protocol.
Note
Result Data
- Applications
Functional Study
Flow Cytometry
- Publication Reference
- The gut microbiota metabolite butyrate mitigates MPTP/MPP+ -induced Parkinson's disease by inhibiting the JAK2/STAT3 signaling pathway.
Li-Li Ji, Ting-Ting Huang, Lun-Lin Mao, Yuan-Feng Xu, Wen-Ya Chen, Wei-Wei Wang, Li-Hui Wang.
The Kaohsiung Journal of Medical Sciences 2023 Oct; 39(10):1002.
Application：Quantitative, Rat, PC12 cells.
- Long non-coding RNA myocardial infarction-associated transcript promotes 1-Methyl-4-phenylpyridinium ion-induced neuronal inflammation and oxidative stress in Parkinson's disease through regulating microRNA-221-3p/ transforming growth factor /nuclear factor E2-related factor 2 axis.
Yue Lang, Hui Zhang, Haojia Yu, Yu Li, Xiao Liu, Minjie Li
Bioengineered 2022 Nov; 13(1):930-940.
- Study on the effect of LncRNA AK094457 on OX-LDL induced vascular smooth muscle cells.
Mei Liu, Yiqun Song, Zhongyuan Han
Am J Transl Res 2019 Oct; 11(9):5623-5633.
- Neuroprotective Effect of Puerarin on Glutamate-Induced Cytotoxicity in Differentiated Y-79 Cells via Inhibition of ROS Generation and Ca(2+) Influx.
Ke Wang, Xue Zhu, Kai Zhang, Zhifeng Wu, Song Sun, Fanfan Zhou, Ling Zhu
Int J Mol Sci 2016 Jul; 17(7):0.
- The gut microbiota metabolite butyrate mitigates MPTP/MPP+ -induced Parkinson's disease by inhibiting the JAK2/STAT3 signaling pathway.