Rex1 (ZFP42) (NM_174900) Human Tagged ORF Clone

Referência RC223916

Tamanho : 10ug

Contactar o distribuidor local :


Telefone :

Rex1 (ZFP42) (NM_174900) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC223916
ZFP42 (Myc-DDK-tagged)-Human zinc finger protein 42 homolog (mouse) (ZFP42)
 
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Rex1
Synonyms REX-1; REX1; zfp-42; ZNF754
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC223916 representing NM_174900
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCAGCAACTGAAGAAACGGGCAAAGACAAGACACCAGAAAGGCCTGGGTGGAAGAGCCCCCAGTG
GGGCTAAGCCCAGGCAAGGCAAGTCAAGCCAAGACCTGCAGGCGGAAATAGAACCTGTCAGCGCGGTGTG
GGCCTTATGTGATGGCTATGTGTGCTATGAGCCTGGCCCTCAGGCTCTCGGAGGGGATGATTTCTCAGAC
TGTTACATAGAATGCGTCATAAGGGGTGAGTTTTCTCAACCCATCCTGGAAGAGGACTCACTTTTTGAGT
CCTTGGAATACCTAAAGAAAGGATCAGAACAACAGCTTTCTCAAAAGGTTTTCGAAGCAAGCTCCCTTGA
ATGTTCTTTGGAATACATGAAAAAAGGGGTAAAGAAAGAGCTTCCACAAAAGATAGTTGGAGAGAATTCG
CTTGAGTATTCTGAGTACATGACAGGCAAGAAGCTTCCGCCTGGAGGAATACCTGGCATTGACCTATCAG
ATCCTAAACAGCTCGCAGAATTTGCTAGAAAGAAGCCCCCCATAAATAAAGAATATGACAGTCTGAGCGC
AATCGCTTGTCCTCAGAGTGGATGCACTAGGAAGTTGAGGAATAGAGCTGCCCTGAGAAAGCATCTCCTC
ATTCATGGTCCCCGAGACCACGTCTGTGCGGAATGTGGGAAAGCGTTCGTTGAGAGCTCAAAACTAAAGA
GACATTTCCTGGTTCATACTGGAGAGAAGCCGTTTCGGTGCACTTTTGAAGGGTGCGGAAAGCGCTTCTC
TCTGGACTTTAATTTGCGTACGCACGTGCGCATCCACACGGGGGAGAAACGTTTCGTGTGTCCCTTTCAA
GGCTGCAACAGGAGGTTTATTCAGTCAAATAACCTGAAAGCCCACATCCTAACGCATGCAAATACGAACA
AGAATGAACAAGAGGGAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC223916 representing NM_174900
Red=Cloning site Green=Tags(s)

MSQQLKKRAKTRHQKGLGGRAPSGAKPRQGKSSQDLQAEIEPVSAVWALCDGYVCYEPGPQALGGDDFSD
CYIECVIRGEFSQPILEEDSLFESLEYLKKGSEQQLSQKVFEASSLECSLEYMKKGVKKELPQKIVGENS
LEYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARKKPPINKEYDSLSAIACPQSGCTRKLRNRAALRKHLL
IHGPRDHVCAECGKAFVESSKLKRHFLVHTGEKPFRCTFEGCGKRFSLDFNLRTHVRIHTGEKRFVCPFQ
GCNRRFIQSNNLKAHILTHANTNKNEQEGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174900
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174900.5
RefSeq Size 2649 bp
RefSeq ORF 933 bp
Locus ID 132625
UniProt ID Q96MM3
Cytogenetics 4q35.2
Protein Families Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell - Pluripotency
MW 34.6 kDa
Summary Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal (By similarity).UniProtKB/Swiss-Prot Function
SKU Description Size
RC223916L1 Lenti ORF clone of Human zinc finger protein 42 homolog (mouse) (ZFP42), Myc-DDK-tagged 10 ug
RC223916L2 Lenti ORF clone of Human zinc finger protein 42 homolog (mouse) (ZFP42), mGFP tagged 10 ug
RC223916L3 Lenti ORF clone of Human zinc finger protein 42 homolog (mouse) (ZFP42), Myc-DDK-tagged 10 ug
RC223916L4 Lenti ORF clone of Human zinc finger protein 42 homolog (mouse) (ZFP42), mGFP tagged 10 ug
RG223916 ZFP42 (tGFP-tagged) - Human zinc finger protein 42 homolog (mouse) (ZFP42) 10 ug
SC306953 ZFP42 (untagged)-Human zinc finger protein 42 homolog (mouse) (ZFP42) 10 ug

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.