Recombinant Human UBA1 Protein (Baculovirus-Insect, His & GST Tag)

Biological Activity

The recombinant human UBA1/GST chimera consists of 1294 amino acids and has a calculated molecular mass of 146 KDa. A DNA sequence encoding the human UBA1 (NP_003325.2) (Ser 2-Arg 1058) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 10% gly, 0.5mM GSH.

Chemical Information

Storage

Stable for up to twelve months from date of receipt at -20℃ to -80℃

References

[1] Marina Ikeda, et al. Biochem J. Herpes simplex virus 1 infection induces ubiquitination of UBE1a

[2] Judith L Green, et al. PLoS Pathog. Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development

[3] Kathrin H Gtz, et al. Cell Chem Biol. Formation of the Alarmones Diadenosine Triphosphate and Tetraphosphate by Ubiquitin- and Ubiquitin-like-Activating Enzymes

[4] Zongyang Lv, et al. Mol Cell. S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity

[5] Ai-Xin Song, et al. PLoS One. A ubiquitin shuttle DC-UbP/UBTD2 reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes