Recombinant Human M-CSF

Macrophage colonystimulating factor (MCSF), also known as CSF1, is a secreted cytokine and key regulator of cellular proliferation, differentiation and survival of blood monocytes, tissue macrophages and their progenitor cells. It is produced by monocytes, fibroblasts and endothelial cells and binds to the CSF1 receptor, also known as CD115. It enhances cytotoxicity, superoxide production, phagocytosis, chemotaxis and secondary cytokine production in monocytes and macrophages (1). MCSF also primes macrophage killing of tumor cells and microorganisms and stimulates pinocytosis. It is known as one of the factors essential for osteoclast development (2). MCSF has been shown to play important roles in modulating dermal thickness and growth of the decidua and placenta during pregnancy (3). It is clinically used in the treatment of infection, malignancies and atherosclerosis, as well as, facilitating hematopoietic recovery after bone marrow transplantation (4).

The biological activity of Human MCSF was determined in a cell proliferation assay using an MCSFdependent murine monocytic cell line, MNFS60 (Halenbeck, R. et al., 1989, Biotechnology 7:710 715). The expected ED₅₀ for this effect is 0.5 1.5 ng/ml.

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Solution

The predicted molecular weight of Recombinant Human MCSF is Mr 18.5 kDa. However, the actual molecular weight as observed by migration on SDSPAGE is Mr 37 kDa.

18.5

This recombinant protein solution was 0.2 µm filtered and formulated in modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.

Working aliquots of this recombinant protein solution are stable for up to twelve months at 20°C to 70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° 8°C for one month without detectable loss of activity. For longterm storage, aliquot and freeze at 20°C to 70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Nemunaitis, J. et al. (1998) Clin. Infect. Dis. 26:1279
2. Ko, JS. et al. (2002) Exp. Mol. Med. 34:340
3. Makrigiannakis, A. et al. (2006) Trends Endicrinol. Metab. 17:178
4. Van de Pol, CJ. et al. (1991) Biotechnol. Therap. 2:231