Recombinant Human Interleukin-31 (E. coli)

Biological Activity

Recombinant Human Interleukin-31 (IL-31) signals through IL-31 receptor A and oncostatin M receptor subunits and can activate STAT3 through receptors and maybe involve in skin immunity. Human IL-31 gene is located on Chr.12. It expresses the IL-31 protein at low levels in the type 2 helper T cells, which exits in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. This protein shares several structural and functional characteristics with IL-6, Oncostatin M, LIF, and Cardiotrophin-1. Human IL-31 regulated immune responses have been implicated in skin physiology and inflammatory skin diseases.

The specific activity is determined by inducing STAT3 activation using human U-87 MG cells. 5 ng/mL of rHuIL-31 can effectively induce STAT3 activation.

Sequence: SHTLPVRLLR PSDDVQKIVE ELQSLSKMLL KDVEEEKGVL VSQNYTLPCL SPDAQPPNNI HSPAIRAYLK TIRQLDNKSV IDEIIEHLDK LIFQDAPETN ISVPTDTHEC KRFILTISQQ FSECMDLALK SLTSGAQQAT T

Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Chemical Information

Solubility (25°C)	Reconstituted in ddH2O or PBS
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Anja Rabenhorst, et al. Curr Allergy Asthma Rep. Interleukin-31: a novel diagnostic marker of allergic diseases

[2] Stacey R Dillon, et al. Nat Immunol. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice