Recombinant Human GH

Referência C725-50ug

Tamanho : 50ug

Marca : Novoprotein

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Description
Recombinant Human Growth Hormone is produced by our E.coli expression system and the target gene encoding Phe27-Phe217 is expressed.
Accession
P01241
Known As
GH1;Somatotropin;Growth hormone;GH;GH-N;Growth hormone 1;Pituitary growth hormone
Mol Mass
22.1 KDa
APMol Mass
20 KDa, reducing conditions
Formulation Description
Lyophilized from a 0.2 μm filtered solution of 20mM HEPES-NaOH, 8% Trehalose,2% Mannitol, 0.1%Tween80, pH7.0.
Endotoxin
< 1 EU/µg as determined by LAL test.
Purity-SDS-PAGE
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Bioactivity-ELISA
Immobilized Recombinant Mouse GHR (C-Fc) (CM02) at 2μg/ml (100 μl/well) can bind Recombinant Human GH(C725)*. *: Biotinylated by NHS-biotin prior to testing.The EC50 of Recombinant Human GH(C725) is 0.70 ng/ml.(Regularly tested)
Reconstitution
  • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
  • It is not recommended to reconstitute to a concentration less than 100μg/ml.
  • Dissolve the lyophilized protein in distilled water.
  • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Shipping
  • The product is shipped at ambient temperature.
  • Upon receipt, store it immediately at the temperature listed below.
Storage
  • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
  • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
  • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Background
Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors. It plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. GH includes prolactin, placental lactogens, proliferins, and somatolactin. It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretory granules. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.