Recombinant Human FSH (HEK293, C-Flag&6His)

Biological Activity

Recombinant Human Follicle-Stimulating Hormone (FSH) is produced by Mammalian expression system and the target gene encoding Ala25-Ser116&Asn19-Glu129 is expressed with a Flag tag&6His at the C-terminus. FSH is a member of glycoprotein hormones subunit beta family, whichalso includes LH, CG and TSH. FSH has a unique β-subunit (FSHβ), which confers its specific biologic activityand is responsible for interaction with the FSH-receptor which belongs to a subfamily of GPCRs calledleucine-rich-repeat-containing GPCRs (LGRs). FSH is secreted from the pituitary gland and regulatesreproduction in mammals. FSH stimulates sertoli cell proliferation in testes and supports spermatogenesis inmales, and induces the maturation of ovarian follicles in females.

Accession: P01215&P01225

APMol Mass: 20-30 KDa, reducing conditions

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Chemical Information

Solubility (25°C)	Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml.
Storage	Stored at ≤ -20°C, stable for one year after receipt

References

[1] Daham Kim, et al. Front Endocrinol (Lausanne). Long-acting recombinant human follicle-stimulating hormone (SAFA-FSH) enhances spermatogenesis

[2] Alireza Khodadadi, et al. Theriogenology. Superovulation of high-producing Holstein lactating dairy cows with human recombinant FSH and hMG

[3] Raoul Orvieto, et al. Front Endocrinol (Lausanne). Optimising Follicular Development, Pituitary Suppression, Triggering and Luteal Phase Support During Assisted Reproductive Technology: A Delphi Consensus

[4] Khalid Abd-Elaziz, et al. Hum Reprod. A new fully human recombinant FSH (follitropin epsilon): two phase I randomized placebo and comparator-controlled pharmacokinetic and pharmacodynamic trials

[5] No authors listed. Hum Reprod Update. Recombinant follicle stimulating hormone: development of the first biotechnology product for the treatment of infertility. Recombinant Human FSH Product Development Group